Process Your Problem Cells
Cryopreserved Live Cells
Cryopreservation is the preferred method to ship and preserve live cells. However, this often leads to lower cell viability and higher debris content upon thawing.
LeviCell EOS processes aid in dead cell and debris removal, yielding improved viability for live single-cell processing.
Frozen Tissue
Single-cell approaches from frozen tissue require nuclei isolation. Existing kits for this process leave behind a lot of debris in downstream processing.
LeviCell EOS nuclei isolation and cleanup procedures remove debris and create a high-quality nuclei suspension.
Barcoded Cells
Cellular barcoding binds a labeled antibody to a cell in order to identify cell surface proteins or support cell hashing or multiplexing. This process leaves behind excess antibodies which are not removed during standard washing steps.
Levitas workflows can remove unbound antibodies, reducing the likelihood of capturing and sequencing these tags and potentially confounding data points.
Benefits of LeviCell EOS
Reduce downtime
Clean 4 samples in ~20 minutes, minimizing transcriptional changes prior to sequencing.
Remove 85% of dead cells
Enrich samples by eliminating debris and dead cells.
Improve cell viability
Move from as low as 20% to as high as 96%.
Be kinder to your cells
Gentle processing preserves fragile cells for sequencing.
